Articles

15N nitrogen labelling on tomato fruit pericarp using plants cultivated in semi-controlled green house conditions

Abstract

Estimating the stability of proteins in plant tissue is an essential but complex question. Today there is very little work showing the feasibility of measuring the turnover of a large panel of plant proteins. Also, we attempted to incorporate labelled nitrogen (15N) into the tomato fruit by adding 5% Ca (15NO3)2 , to the conventionally used nutrient solution. One month of culture later (with anthesis notation), four 15N incorporation kinetics were performed on fruits at different stages of development during 11 days. Assays of isotopic ratios carried out by EA-IRMS (Elementary Analysis - Isotopic Ratio by Mass Spectrometry) on insoluble residues after hydro-alcoholic extraction show a good in corporation of the labelling. Combined with total protein assays (Bradford), the results reveal the perfect linearity of 15N enrichment in tomato pericarp during cell expansion phase.

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Authors


Marie-Hélène Andrieu

marie-helene.andrieu@inrae.fr

https://orcid.org/0000-0001-9616-3426

Affiliation : UMR 1332 - Biologie du fruit et Pathologie INRAE- Domaine Grande Ferrade, 33140 Villenave d’Ornon, France

Country : France


Isabelle Atienza

Affiliation : UMR 1332 - Biologie du fruit et Pathologie INRAE- Domaine Grande Ferrade, 33140 Villenave d’Ornon, France.

Country : France


Cédric Cassan

https://orcid.org/0000-0003-3022-4278

Affiliation : UMR 1332 - Biologie du fruit et Pathologie INRAE- Domaine Grande Ferrade, 33140 Villenave d’Ornon, France.

Country : France


Anne Marmagne

https://orcid.org/0000-0003-3466-9477

Affiliation : UMR1318 - Institut J-P Bourgin – INRAE, AgroParisTech, Université Paris Saclay, 78000, Versailles, France.

Country : France


Daniel Just

https://orcid.org/0000-0002-3265-4696

Affiliation : UMR 1332 - Biologie du fruit et Pathologie INRAE- Domaine Grande Ferrade, 33140 Villenave d’Ornon, France.

Country : France


Willy Bienvenut

https://orcid.org/0000-0003-4192-3920

Affiliation : UMR GQE - Équipes PAPPSO/BASE (UPS, INRAE, CNRS, AgroPTech), Le Moulon, 91190 Gif-sur-Yvette, France.

Country : France


Yves Gibon

https://orcid.org/0000-0001-8161-1089

Affiliation : UMR 1332 - Biologie du fruit et Pathologie INRAE- Domaine Grande Ferrade, 33140 Villenave d’Ornon, France

Country : France


Sophie Colombié

Affiliation : UMR 1332 - Biologie du fruit et Pathologie INRAE- Domaine Grande Ferrade, 33140 Villenave d’Ornon, France

Country : France

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