Articles
15N nitrogen labelling on tomato fruit pericarp using plants cultivated in semi-controlled green house conditions
Published : 1 June 2022
Abstract
Estimating the stability of proteins in plant tissue is an essential but complex question. Today there is very little work showing the feasibility of measuring the turnover of a large panel of plant proteins. Also, we attempted to incorporate labelled nitrogen (15N) into the tomato fruit by adding 5% Ca (15NO3)2 , to the conventionally used nutrient solution. One month of culture later (with anthesis notation), four 15N incorporation kinetics were performed on fruits at different stages of development during 11 days. Assays of isotopic ratios carried out by EA-IRMS (Elementary Analysis - Isotopic Ratio by Mass Spectrometry) on insoluble residues after hydro-alcoholic extraction show a good in corporation of the labelling. Combined with total protein assays (Bradford), the results reveal the perfect linearity of 15N enrichment in tomato pericarp during cell expansion phase.
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