Allele-specific PCR: development and application to SNP genotyping in Californian thrips

Abstract

In this study we present a technique of ADN extraction adaptated to very small samples like the californian thrips as well as the implementation and the genotyping by allele-specific PCR of G275E of the sub-unit a6 of the nicotine receptor of the studied acetylcholine, from this pest, in the resistance to a biological insecticide : Spinosad. The double objective of this technique is to be able to genotype rapidly, economically and individually insects that are very difficult to see and to be able to create a distribution map of the G275E among French population of Frankliniella occidentalis.