Articles
Preservation of viable and functional plant-associated microbial communitias: first results
Abstract
Microbiota are communities of microorganisms living associated with all living beings. They are of a great interest in agriculture, including to enhance plant health. However, their handling requires to preserve them alive and functioning on a long-term basis. Reliable preservation of these communities is not simple, because they are composed of a great diversity of microorganisms, some of them we do not know how to cultivate. At the French Collection for Plant-associated Bacteria (CIRM-CFBP) located at INRAE Angers, we proposed to measure the impact of the preservation on microbiota associated with radish leaves and seeds. We measured the impact of preservation on the taxonomic diversity (by barcoding) and the functional diversity (by Biolog technology) of these microbiotas before and after preservation for two years. This work enhances knowledge of the influence of preservation and of a multiplication stage after preservation. It will permit to make tools available to scientists enancing the preservation of these resources.
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