Articles
Determining cellular and subcellular volumes by analyzing microscope images: How can the speed of fruit cell growth be estimated?
Published : 1 December 2023
Abstract
The estimation of cell volumes and subcellular proportions is necessary for studying metabolic fluxes in fruit during its development. By analyzing optical microscopy images using the freeware "Image J", we were able to collect the data needed to parameterize a fruit cell model described as ellipsoidal in shape. The volume estimates obtained for different stages of fruit development enabled us to obtain growth kinetics for the average cell and its major compartments. By fitting the measured data to an appropriate mathematical function, we were able to calculate cell growth rate over time
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