Preparations of lignocellulosic material, observation cells and associated constraints for multi-technique imaging of enzymatic degradation

Abstract

Various preparations of sample sections have been tested to observe enzyme localization without any previous labelling, some vegetal wall components and their evolution during the degradation. Preparation of paraffin sections has been chosen to obtain spectral and hyperspectral images in real time. Two methods of closely related and complementary imaging were set out : - fluorescence microscopy with excitation in deep UV allows to obtain some information about the location of enzymes by multi-scale imaging - FT-IR microspectroscopy with the use of microfluid cell allows to follow in real time the cell wall component change under enzyme action.