Plant genomic DNA extraction technique

Abstract

The extraction of genomic DNA from a small quantity of vegetal material is commonly made/practiced by means of
commercial kits, which have the advantages to be rapid, reproductible and toxic solvent-free. These commercial kits are effective when the obtained samples are used to amplify by PCR (polymerase chain reaction) small fragments (<1 kb). As we have to amplify by PCR a large fragment of DNA (> 3kpb), we set up a protocol described in this paper that allows us to extract from small strands of vegetal material a DNA of high molecular weight. Our results show that the expected PCR amplification can be made on our samples and that their shelf-life lasts long. We also show that this protocol can be applied on other vegetal species. Moreover, the cost per sample is reduced in comparison with an extraction made with a laboratory supplier kit.